Zhonghua Bing Li Xue Za Zhi 2000 Dec;29(6):435-8 Related Articles, Links


[Cerulenin inhibits growth of human colonic carcinoma in nude mice]

[Article in Chinese]

Huang P, Zhu S, Lu S, Li L, Dai Z, Jin Y.

Department of Pathology, Medical College, Southeast University, Nanjing 210009, China.

OBJECTIVE: To determine the effects of fatty acid synthase inhibitor, cerulenin, on tumor growth of human colonic carcinoma (LoVo) in nude mice. METHODS: The transplantable colonic carcinoma cell line LoVo model was established in BALB/CA-nu/nu mice by subcutaneous implantation. Cerulenin was administrated by i.p. injection (80 mg/kg, 160 mg/kg each time) for 10 times. 17 days later the mice were killed and tumor size evaluated. RESULTS: The size of tumors in cerulenin-treated group were significantly smaller than in control group and were similar to that of the 5-Fu treated group. Transmission electronic microscopy (TEM) revealed that death of LoVo cells treated by cerulenin was associated with apoptosis characterized by cell shrinkage, condensation and chromosome fragmentation. bcl-2 oncoprotein expression rate was significantly lower in cerulenin-treated group in comparing with the control group. The expression rate of bax was the opposite to that of bcl-2. CONCLUSIONS: Cerulenin effectively suppresses the growth of human colonic carcinoma cells in vivo. The mechanism of cell death may be correlated with apoptosis, and bcl-2 and bax gene may play an important role in regulating cerulenin-induced apoptosis.

PMID: 11866947



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